Biological Nitrogen Removal Database

A manually curated data resource for microbial nitrogen removal


Detailed information

Microorganism

Pseudomonas putida strain AD-21

Taxonomy
  • Phylum : Proteobacteria
  • Class : Gammaproteobacteria
  • Order :Pseudomonadales
  • Family : Pseudomonadaceae
  • Genus : Pseudomonas

Electron Acceptor

NO3-,NO2-

Electron Donor

Various compounds

Information about Article

Reference:Kim et al, 2008

Title:Aerobic Denitrification of Pseudomonas Putida AD-21 at Different C/N Ratios

Pubmed ID:19111647

Pubmed link:Link

Full research link:Link

Abstract: An aerobic denitrifier was newly isolated from soil and its denitrification activity under different C/N (carbon/nitrogen) ratios was investigated. The isolate was identified as Pseudomonas putida AD-21 by biochemical studies and 16s rDNA sequencing analysis. P. putida AD-21 tolerated oxygen levels of 5.0-6.0 mg/l. An (15)NH(4)/(14)NO(3) analysis indicated that the nitrogen of NH(4) was preferentially assimilated into the cell mass and that the nitrate removed could be considered an indication of bacterial denitrification efficiency. Increasing the C/N ratio increased the nitrate removal rates, whereas nitrogen assimilation into the cell mass was not affected. The optimal C/N ratio was 8 with a maximum nitrate removal rate of 254.6 mg/l/h and a nitrate removal efficiency of 95.9%. The results suggest that P. putida AD-21 may be a good candidate for aerobic wastewater treatment.

Biological Nitrogen Removal Database